Expression of Batis maritima methyl chloride transferase in Escherichia coli

Expression of Batis maritima methyl chloride transferase in Escherichia coli.

Methyl chloride transferase, a novel enzyme found in several fungi, marine algae, and halophytic plants, is a biological catalyst responsible for the production of atmospheric methyl chloride. A previous paper reports the purification of this methylase from Batis maritima and the isolation of a cDNA clone of the gene for this enzyme. In this paper, we describe the isolation of a genomic clone o...

cDNA cloning of Batis maritima methyl chloride transferase and purification of the enzyme.

Methyl chloride transferase catalyzes the synthesis of methyl chloride from S-adenosine-L-methionine and chloride ion. This enzyme has been purified 2,700-fold to homogeneity from Batis maritima, a halophytic plant that grows abundantly in salt marshes. The purification of the enzyme was accomplished by a combination of ammonium sulfate fractionation, column chromatography on Sephadex G100 and ...

Advances and challenges in recombinant protein expression in Escherichia coli

Significance of physicochemical factors in the transmission of Escherichia coli and chloride

Background: Organic manures are the source of many pathogenic bacteria which could be dangerous for human health. Bacterial transmission and retention in soil is important for processes ranging from contaminant degradation during in situ bioremediation to transport of pathogenic bacteria into groundwater. Methods: The aim of this study was to evaluate the transport of Escherichia coli and chl...

Expression in Escherichia coli

The gene for protein D, a membrane-associated protein with specific affinity for human immunoglobulin D, was cloned from a nontypeable strain of Haemophilus influenzae. The gene was expressed in Escherichia coli from an endogenous promoter, and the gene product has an apparent molecular weight equal to that of H. influenzae protein D (42,000). The complete nucleotide sequence of the gene for pr...